| Sharps (needles, syringes, scalpel blades, etc.) |
- Place intact sharps, whether contaminated or not, in a puncture resistant "sharps" container.
- Do no recap, bend, remove, or clip needles.
- Fill 3/4 full, snap the lid close and secure with tape. Do Not Overfill.
- Complete and submit a Hazardous & Regulated Waste Removal Request Form to EHS.
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| Non-sharp Solid Waste for Autoclave |
- Collect in autoclavable bags without the word "biohazardous" or the universal symbol for biohazardous material; clear or white bags are preferable.
- Place filled bags into an autoclave PL pan for transport from the laboratory to the autoclave.
- Add the appropriate amount of water to the bag and close loosely to allow steam to escape.
- Autoclave the pan and bag at 121 degrees Celsius for one hour.
- Allow the pan and autoclaved material to cool; put the autoclaved material in the building dumpster.
- Do not autoclave chemical or radioactive wastes.
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| Contaminated Glass and Pasteur Pipets |
Treat by one of the following two methods:
- Decontaminate the glass by autoclaving, or soaking in 10% bleach for 30 minutes, then place in a sturdy cardboard box, tape closed, and dispose of in the building dumpster; or,
- Contaminated glass may be discarded into a sharps container and handled according to the instructions for "Sharps."
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| Liquid Waste |
- Add household bleach to a final concentration of 10%, wait 30 minutes, then rinse down the sink with copious amounts of water.
- Alternatively, a disinfectant that is known to be effective against the organism may be added to an appropriate concentration, wait 30 minutes, then rinse down the sink with copious amounts of water.
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| Animal Carcasses |
- Place animal carcasses/tissues into a plastic bag. Doublebag all carcasses when zoonotic agents are present.
- Place bag in freezer until removal.
- Disposal of animal carcasses/tissues is coordinated through the IACUC.
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